Product: Anti-Cathepsin B mAb, clone CB131
Cat. No.: MC-130 (1 mL, lyophilized)
Background:
Cathepsin B is a lysosomal cysteine protease
expressed in a variety of different types of
normal and neoplastic tissues. It may be
associated with plasma membranes in many
solid organ malignant tumors. Cathepsin B may
be expressed in stromal cells, including
macrophages and fibroblasts, of neoplastic
tissue even where the tumor is negative.
Enzymatic studies have revealed cathepsin B in
many normal human tissues including skeletal
muscle, liver, heart and adrenal glands.
Immunohistochemical expression has been
detected in gut mucosa, skin, prostate, thyroid
with weak expression in brain. Cathepsin B has
been demonstrated in human and rat heart,
where it degrades acute ischaemic myocardial
cells. Cathepsin B has been reported in breast
tumors as well as in normal breast epithelium. A
number of endothelial cells are positive in many
tissues where these have been described as
sprouting endothelial cells and this is thought to
be related to tumor progression. Cathepsin B is
over-expressed in squamous cell carcinoma
where undifferentiated cells are strongly positive
and the more differentiated cells in tumor islands
are either weakly positive or negative. The
expression of cathepsin B has also been noted
in melanomas where the upregulation of this
enzyme was found to be a characteristic of a
more invasive tumor phenotype.
Species Reactivity:
Human
Ig Isotype:
Mouse IgG2b
Immunogen:
Prokaryotic recombinant protein corresponding to
the full length mature human cathepsin B
molecule.
Staining pattern:
Cytoplasmic
Format:
1 mL lyophilized tissue culture supernatant
containing 15 mM sodium azide.
Positive control:
Skin
Reconstitution:
Reconstitute with 1 mL sterile distilled water.
Storage:
Unopened lyophilized antibody can be stored at
4°C. After reconstitution, the antibody is stable for
at least two months when stored at 4°C. For long
term storage, it is recommended that aliquots of
the antibody be made and frozen at -20°C (frostfree
freezers are not recommended). Repeated
freezing and thawing must be avoided. Prepare
working dilutions on the day of use.
Applications:

Immunohistochemistry: Paraffin sections.
Use at a 1:40 - 1:80 dilution. Use a 60
minute primary antibody incubation at 25°C.
Standard ABC technique.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product