Product: Anti-CARD10 pAb
Cat. No.: PC-526 (100 μg)
Synonyms:
Anti-Caspase Recruitment Domain Family 10,
anti-CARMA3, anti-Bimp1.
Background:
Apoptosis is related to many diseases and
development. Cell death signals are transduced
by death domain (DD), death effector domain
(DED), and caspase recruitment domain (CARD)
containing molecules. CARD containing proteins
include some caspases, Apaf-1, CARD4, IAPs,
RICK, ARC, RAIDD, Bcl-10, and ASC. A novel
CARD-containing protein was recently identified
and designated CARD10. This protein belongs
to the membrane-associated guanylate kinaselike
(MAGUK) family of proteins that can function
as molecular scaffolds that assist assembly of
signal transduction molecules. CARD 10
interacts with Bcl-10, a Bcl protein that promotes
apoptosis, caspase-9 maturation and activation
of NF-
B. CARD 10 and the related protein
CARMA1 also associate with NEMO, the
regulatory subunit of the I
K complex,
demonstrating its importance in the regulation of
NF-
B transcription factor activation.
Specificity:
Recognizes CARD10.
Positive Control:
EL4 cell lysates.
Species Reactivity:
Human and mouse. Other species not tested.
Host:
Rabbit
Immunogen:
A synthetic peptide corresponding to 14 amino
acids at the C-terminus of human CARD10
(Genbank accession No. AAK26165).
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Application and Suggested Dilution:

Western blot: Use at 5 - 10 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure: Western blot analysis of CARD10 expression
in EL4 cell lysate with anti-CARD10 pAb at (A) 5
μg/mL and (B) 10 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.